•Prefer
if K is constant over conc. range
•If
not constant we can work in a narrow range where it is
constant
•This
is linear chromatography
•
•Example
of non-linear chromatography
–Pour
aqueous solution of A & B on column
–Allow
water to drain out, both A & B stick
–Wash
column with 50% MeOH, A removed
–Wash
column with 100% MeOH, B removed
•Used
extensively for sample cleanup in GC