•More useful relationships - capacity factor k’
•(comes from K) K in concentration, k’ in moles
•
•           amount of analyte in stationary phase
n   k’ = ----------------------------------------------
n             amount of analyte in mobile phase
n
n                                   KAVs          ns            n = # of
nSo for A  à    kA’  =  ----------  =  ------         moles
n                                     VM           nM
                     1
 n = µ x ------------------
               1 + K Vs/VM
                  1
 n = µ x -----------
                1 + kA’
From previous slide