•Solvents (mobile phase) – are stored

•in special reservoirs connected to the

•pumping system – must be free of

•particles that can clog components & free of

•bubble forming gases that get trapped in

•column or detector

•Three basic ways to degas solvents

• 1) vacuum or suction filter (0.4 or 0.2 µm)

• 2) ultrasonicate (with vacuum)

• 3) He purge (sparge units often built in)

•Can purchase HPLC solvents & water - still