•Size Exclusion Chrom. (SEC)
•Packings are porous polymeric (resins) or silica based
materials
•Two names used for the same process:
•1) Gel
filtration chrom. = aqueous solvent
2)Gel permeation
chromatography = non-aqueous mobile phase
•Column packing works like a molecular filter allowing
small molecules access to every pore, retarding their
progress – large molecules pass thru more quickly