Quantitative Aspects of Absoption
•Beer-Lambert Law (or Beer’s Law)
•
•                                  Io
•                  A  =  log ----  =  ε b C
•                                  I
•
•                 I
•       T  =  ----           %T  =  T x 100
•                 Io
•
•Io  =  measured source intensity
•
•I  =  measured intensity after absorption
•
•Intensity change does not change absorbance
Absorbance
Transmittance
molar absorptivity
concentration
path length