Effects other than absorption that reduce source
intensity (i.e., scattering, reflection) may also be measured as
absorbance and must be accounted for when measuring I & Io
Incident
Beam
Reflection
Loses
Reflection
Loses
Cuvette
Transmitted
Beam
Light
loses occur due to:
1)reflection at boundaries
2)scattering by molecules
or particles
3)
absorption which is
process of interest
scatter